Ibintu bikurura abantu cyaneDMPTnaDMTni ibintu bishya kandi byiza gukurura inyamaswa zo mu mazi. Muri ubu bushakashatsi, ibintu bikurura inyamaswa zo mu mazi bifite imbaraga nyinshiDMPTnaDMTbyongewe ku biryo bya carp kugira ngo harebwe ingaruka z'ibikurura byombi ku kugaburira carp no guteza imbere imikurire. Ibisubizo byagaragaje ko hongewemo ibikurura bikomeyeDMPTnaDMTku biryo byongereye cyane inshuro zo kuruma amafi yageragejwe kandi bigira ingaruka zikomeye ku kugaburira; Muri icyo gihe, kongeramo ibintu bitandukanye bikurura amafi cyaneDMPTnaDMTku biryo byongera cyane igipimo cyo kwiyongera k'ibiro, igipimo cyihariye cyo gukura, n'igipimo cyo kubaho kw'amafi yo mu igerageza, mu gihe igipimo cy'ibiryo cyagabanutse cyane. Ibisubizo by'ubushakashatsi kandi bigaragaza koDMPTbigira ingaruka zikomeye mu gukurura no guteza imbere ikura rya carp ugereranije naDMT.

Igikurura amatungo mu mazi ni inyongeramusaruro idatanga intungamubiri. Kongeramo ibikurura amatungo mu kugaburira amafi bishobora gutuma arya neza, byongera ibyo arya, bikagabanya ibisigaye mu mazi, bityo bigabanye umwanda mu mazi yororerwamo.DMPTnaDMTNi ibintu bikora biboneka cyane mu binyabuzima byo mu mazi, bitanga methyl neza kandi bikaba ari ibintu by'ingenzi bigenzura umuvuduko wa osmotic. Bifite kandi ingaruka zikomeye ku nyamaswa zo mu mazi kugira ngo zigere ku byo zirya kandi zikure.

Nyuma yo gukora ubushakashatsi ku nyamaswa zo mu mazi nka crucian carp, red snapper, goldfish, na spotted shrimp, abashakashatsi b’Abayapani basanzeDMPTnaDMTbigira ingaruka nziza ku mafi yo mu mazi meza n'ayo mu mazi, inyamaswa zo mu bwoko bwa crustacean n'ibinyamisogwe. Yongeraho ingano nto y'ibintu bikurura abantu cyaneDMPTnaDMTmu biryo bishobora kwihutisha cyane kugaburira no gukura kw'amafi atandukanye yo mu mazi meza n'ayo mu mazi. Muri ubu bushakashatsi, ibintu bikurura amafi cyaneDMPTnaDMTbyongewe ku biryo bya carp kugira ngo byige ingaruka zabyo ku kugaburira carp no guteza imbere iterambere, bitanga amakuru y’ibanze ku ikoreshwa ryagutse ry’ibi biryo bibiri bishya bikurura abantu mu nganda z’ibiryo n’ubworozi bw’amafi.

1 Ibikoresho n'Uburyo

1.1 Ibikoresho byo kugerageza n'amafi yo kugerageza
S. S' - Dimethylacetic aside thiazole (DMT), DMPT
Ifi zo mu bwoko bwa carp zavuye mu bworozi bw’amafi, zifite imibiri myiza n’imiterere myiza. Mbere y’uko igerageza ritangira ku mugaragaro, amafi yo mu bwoko bwa carp azarorerwa by’agateganyo muri laboratwari mu gihe cy’iminsi 7, aho azagaburirwa ibiryo by’ifi zo mu bwoko bwa carp byatanzwe n’uruganda rubigaburira.
1.2 Ifunguro ry'igerageza
1.2.1 Ibiryo byo gupima urushundura: Shyira ibiryo bya carp bitangwa n'uruganda rubigaburira, ongeramo ingano ingana ya A-starch, uvange neza, hanyuma uvange n'amazi meza akwiye kugira ngo ukore udupira dufatana twa 5g buri kimwe nk'ibiryo by'itsinda rigenzura. Muri icyo gihe, tegura ibiryo by'ibiryo ubanje gusya ibiryo bya carp, wongeremo ingano ingana ya alpha starch, hanyuma wongereho DMT naDMPTku gipimo cya kabiri cya 0.5g/kg na 1g/kg, uko bikurikirana. Vanga neza hanyuma uvange n'amazi meza akwiye kugira ngo buri garama 5 z'umugati ufatane.
1.2.2 Ibiryo bipima gukura:

Shyira ibiryo bya karpi (biva ku isoko imwe n'iyavuzwe haruguru) mu ifu, ubinyujije mu kayunguruzo ka mesh 60, ongeramo ingano ingana ya alpha starch, uvange neza, uvange n'amazi yaciwe, ubikuremo mu kayunguruzo ubishyiremo utubumbe, hanyuma ubyumishe mu mwuka kugira ngo ubone ibiryo by'itsinda rigenzura kugira ngo bigere ku gipimo cyo gukura.DMTna kristu za DMPT zashongeshejwe mu mazi yaciwe kugira ngo hategurwe umuti w’ingano ikwiye, wakoreshejwe mu kuvanga ibiryo bya karpi bivanze neza n’ibinyampeke mu tunyangingo. Nyuma yo kumisha, ibiryo by’itsinda byakoreshejwe mu bushakashatsi byabonetse, hamweDMTna DMPT yongewemo ibice bitatu bya 0.1g/kg, 0.2g/kg, na 0.3g/kg, uko bikurikirana.

1.3 Uburyo bwo Gupima
1.3.1 Ikizamini cy'urushundura: Hitamo ifi 5 zo mu bwoko bwa carp zo mu igerageza (zifite uburemere bwa garama 30) nk'ifi yo mu igerageza. Mbere yo gukora ikizamini, shyira ifi yo mu igerageza mu ikirahuri cya aquarium (ifite ingano ya cm 40 × 30 × 25). Ibiryo byo mu igerageza bishyirwa ku ntera ya cm 5.0 uvuye hasi mu ishyamba ukoresheje umurongo uhambiriye ku rubaho rutambitse. Ifi iruma icyo gishundura ikanyeganyega, gihita cyoherezwa ku rubaho rutambitse kandi kigafatwa n'icyuma gifata amapine. Inshuro zo kurya icyo gishundura zibarwa hashingiwe ku kuntu amafi 5 yo mu igerageza yanyeganyega cyane mu minota 2. Ikizamini cyo kurya kuri buri tsinda ry'ibiryo cyasubiwemo gatatu, hakoreshejwe udupira dushya two kugaburira buri gihe. Mu gukora igerageza risubiwemo kugira ngo haboneke umubare wose n'inshuro zo kurya, ingaruka zo kugaburiraDMTna DMPT kuri carp ishobora gupimwa.

1.3.2 Igerageza ryo gukura rikoresha utuzu 8 tw’amazi (ingano ya 55 × 45 × 50cm), dufite ubujyakuzimu bw’amazi bwa cm 40, ubushyuhe bw’amazi karemano, n’izamuka ry’amazi rihoraho. Amafi yakoreshejwe mu igerageza yashyizwe mu matsinda abiri mu buryo butunguranye kugira ngo akoreshwe. Itsinda rya mbere rigizwe n’utuzu 4 tw’amazi, dufite inomero X1 (itsinda rigenzura), X2 (ibiryo 0.1gDMT/kg), X3 (ibiryo 0.2gDMT/kg), X4 (ibiryo 0.3gDMT/kg); Irindi tsinda ry’utuzu 4 tw’amazi, dufite inomero Y1 (itsinda rigenzura), Y2 (ibiryo 0.10g DMPT/kg), Y3 (ibiryo 0.2g DMPT/kg), Y4 (ibiryo 0.30g DMPT/kg). Amafi 20 kuri buri gasanduku, agaburirwa inshuro 3 ku munsi saa 8:00, 13:00, na 17:00, hamwe n’umuvuduko wo kugaburira buri munsi wa 5-7% by’uburemere bw’umubiri. Igerageza ryamaze ibyumweru 6. Mu ntangiriro no mu mpera z'igerageza, uburemere bw'ifi y'igerageza bwapimwe kandi igipimo cyo kubaho kwa buri tsinda cyanditswe.

2.1 Ingaruka zo kugaburira za DMPT naDMTkuri karpi
Ingaruka zo kugaburira za DMPT naDMTku ifi ya karpufe bigaragazwa n'uburyo amafi yo mu igerageza yarumaga mu gihe cy'igerageza ry'iminota 2, nk'uko bigaragara ku mbonerahamwe ya 1. Igerageza ryagaragaje ko nyuma yo kongeramo ibiryo bya DMPT na DMT mu ruziga, amafi yo mu igerageza yagaragaje imyitwarire yo gushaka ibyo kurya vuba, mu gihe iyo bakoreshaga ibiryo by'itsinda ry'abagenzura, uburyo amafi yo mu igerageza yakiraga bwatinze cyane. Ugereranyije n'ibiryo byo kugenzura, amafi yo mu igerageza yagize ubwiyongere bugaragara bw'inshuro zo kuruma ibiryo byo mu igerageza. DMT na DMPT bigira ingaruka zikomeye ku igerageza rya karpufe.

Igipimo cyo kwiyongera ibiro, igipimo cy’ubwiyongere bwihariye, n’igipimo cyo kubaho kw’inyama zo mu bwoko bwa carp zigaburirwa hamwe n’ibipimo bitandukanye bya DMPT byiyongereye cyane ugereranije n’izo zigaburirwa hamwe n’ibiryo bigenzurwa, mu gihe igipimo cy’ibiryo cyagabanutse cyane. Muri byo, kongeramo DMPT kuri T2, T3, na T4 byongereye ibiro bya buri munsi by’amatsinda atatu ku kigero cya 52.94%, 78.43%, na 113.73%, ugereranije n’itsinda rigenzurwa. Igipimo cyo kwiyongera ibiro cya T2, T3, na T4 cyiyongereyeho 60.44%, 73.85%, na 98.49%, uko bikurikirana, kandi igipimo cy’ubwiyongere cyihariye cyiyongereyeho 41.22%, 51.15%, na 60.31%, uko bikurikirana. Igipimo cyo kubaho cyiyongereye kuva kuri 90% kigera kuri 95%, naho igipimo cy’ibiryo cyagabanutseho 28.01%, 29.41%, na 33.05%.

3. Umwanzuro

Muri iki gikorwa, nibaDMTcyangwa DMPT yongeweho, inshuro zo kugaburira, igipimo cyihariye cy’ikura, n’ubwiyongere bw’ibiro bya buri munsi by’amafi yo kugerageza muri buri tsinda byiyongereye cyane ugereranije n’itsinda ry’abagenzurwa, mu gihe igipimo cy’ibiryo cyagabanutse cyane. Kandi yaba DMT cyangwa DMPT, ingaruka zo guteza imbere ikura ziba zikomeye cyane hamwe n’ubwiyongere bw’ingano y’inyongera mu byiciro bitatu bya 0.1g/kg, 0.2g/kg, na 0.3g/kg. Muri icyo gihe, hakozwe igereranya ry’ingaruka zo kugaburira no gutera ikura za DMT na DMPT. Byagaragaye ko munsi y’ubwiyongere bumwe bw’imisatsi, inshuro zo kugaburira, igipimo cyo kwiyongera kw’ibiro, n’igipimo cyihariye cy’ikura ry’amafi yo kugerageza mu itsinda ry’ibiryo bya DMPT byiyongereye cyane ugereranije n’itsinda ry’ibiryo bya DMT, mu gihe igipimo cy’ibiryo cyagabanutse cyane. Mu buryo bunyuranye, DMPT ifite ingaruka zikomeye mu gukurura no guteza imbere ikura rya carp ugereranije na DMT. Iri gerageza ryakoresheje DMPT na DMT byongewe ku biryo bya carp kugira ngo harebwe ingaruka zo kugaburira no gutera ikura. Ibisubizo bigaragaza ko DMPT na DMT bifite amahirwe menshi yo gukoreshwa nk’ubwoko bushya bw’inyamaswa zo mu mazi zikurura.