Imbaraga zikururaDMPTnaDMTni bishya kandi bikurura inyamaswa zo mu mazi. Muri ubu bushakashatsi, imbaraga zikurura imbaragaDMPTnaDMTbyongewe kubiryo bya karp kugirango bikore iperereza ku ngaruka zabakurura bombi ku kugaburira karp no kuzamura iterambere. Ibisubizo byerekanaga ko kongeramo imbaraga-zikomeye zikururaDMPTnaDMTkugaburira byongereye cyane inshuro yo kuruma amafi yubushakashatsi kandi byagize ingaruka zikomeye zo kugaburira; Mugihe kimwe, hiyongereyeho ibitekerezo bitandukanye byimbaraga zikomeye zikururaDMPTnaDMTkugaburira byongereye cyane umuvuduko wo kongera ibiro, umuvuduko wubwiyongere bwihariye, nigipimo cyo kubaho kwamafi yubushakashatsi, mugihe coefficient yibiryo yagabanutse cyane. Ibisubizo by'ubushakashatsi nabyo birerekana koDMPTifite ingaruka zikomeye mukureshya no guteza imbere imikurire ya carp ugereranijeDMT.

Ibiryo bikurura inyamaswa zo mu mazi ni inyongeramusaruro. Ongeraho ibikurura kugaburira amafi birashobora guteza imbere neza kugaburira kwabo, kongera ibiryo byabo, kugabanya ibiryo bisigaye mumazi, bityo bikagabanya umwanda mumazi y’amazi y’amafi.DMPTnaDMTni ibintu bifatika biboneka mu binyabuzima byo mu nyanja, bikora nk'abaterankunga ba methyl neza kandi bigenzura ingufu za osmotic. Bafite kandi kugaburira no gukura bigira ingaruka ku nyamaswa zo mu mazi.

Abashakashatsi b'Abayapani nyuma yo gukora ubushakashatsi bujyanye n’inyamaswa zo mu mazi nka karipi yabambwe, snapper itukura, amafi ya zahabu, hamwe na shrimp iboneka.DMPTnaDMTbigira ingaruka nziza zikurura amazi meza n amafi yo mu nyanja, crustaceans, na shellfish. Kuzuza imbaraga nke zabakurura imbaraga nyinshiDMPTnaDMTmubiryo birashobora kwihutisha cyane kugaburira no gukura kwamazi meza n amafi yo mu nyanja. Muri ubu bushakashatsi, imbaraga zikurura imbaragaDMPTnaDMTbyongewe kubiryo bya karpi kugirango bige ingaruka zabyo mukugaburira karp no kuzamura iterambere, bitanga amakuru yerekeranye no gukwirakwiza kwinshi kwaba bakurura bashya mu nganda n’ibihingwa by’amafi.

1 Ibikoresho nuburyo

1.1 Ibikoresho byubushakashatsi n amafi yubushakashatsi
S. S '- Dimethylacetic aside thiazole (DMT), DMPT
Carp yubushakashatsi yakuwe mu murima w’amafi, ifite umubiri muzima kandi usobanutse neza. Mbere yuko igeragezwa ritangira kumugaragaro, amafi yubushakashatsi azazamurwa byigihe gito muri laboratoire muminsi 7, mugihe bazagaburirwa ibiryo bya karpi bitangwa nuruganda rugaburira.
1.2 Ibiryo by'igeragezwa
1.2 Muri icyo gihe, tegura ibiryo byokurya ubanza guhonda ibiryo bya karp, wongeremo ingano ingana ya alfa, hanyuma wongereho DMT naDMPTkuri concentration ebyiri za 0.5g / kg na 1g / kg, kimwe. Kuvanga neza hanyuma ukavanga namazi akwiye yamazi yatoboye kugirango buri mupira 5g ufate.
1.2.2 Ibiryo byo gukura bikura:

Kumenagura ibiryo bya karpi (biva mumasoko amwe nkuko byavuzwe haruguru) mubifu, ubinyujije mumashanyarazi ya mesh 60, ongeramo ingano ingana na alfa ya krahisi, vanga neza, uvange n'amazi yatoboye, uyikure mumashanyarazi muri granules, hanyuma umwuka uyumishe kugirango ubone ibiryo byitsinda rishinzwe kugenzura ikizamini cyo gukura. GukomatanyaDMTna kirisiti ya DMPT yashongeshejwe mumazi yatoboye kugirango hategurwe igisubizo cyibitekerezo bikwiye, byakoreshwaga mu kuvanga ibiryo bya karpu bivanze neza hamwe na krahisi muri granules. Nyuma yo kumisha, ibiryo by'itsinda ryageragejwe byabonetse, hamweDMTna DMPT yongewemo ibintu bitatu byibanze bya 0.1g / kg, 0.2g / kg, na 0.3g / kg.

1.3 Uburyo bwo Kwipimisha
1.3.1 Ikizamini cyamayeri: Hitamo karp 5 yubushakashatsi (ufite uburemere buringaniye bwa 30g) nkamafi yikizamini. Mbere yikizamini, inzara amasaha 24, hanyuma ushire amafi yikizamini muri aquarium yikirahure (gifite ubunini bwa 40 × 30 × 25cm). Ibiryo bikurura byashyizwe ku ntera ya 5.0cm uhereye hepfo ya aquarium ukoresheje umurongo wahagaritswe uhambiriye umurongo utambitse. Ifi iruma ibyambo kandi iranyeganyega umurongo, woherezwa mukibari gitambitse kandi cyandikwa nicyuma gifata amajwi. Inshuro yo kuruma ibyambo bibarwa hashingiwe ku kunyeganyega kw'amafi 5 yipimishije kuruma ibyambo mu minota 2. Ikizamini cyo kugaburira kuri buri tsinda ryibiryo cyasubiwemo inshuro eshatu, ukoresheje imipira mishya yo kugaburira buri gihe. Mugukora ubushakashatsi bwisubiramo kugirango ubone umubare rusange hamwe nimpuzandengo yinshyi yo kuroba, ingaruka zo kugaburira zaDMTna DMPT kuri carp irashobora gusuzumwa.

1.3.2 Ubushakashatsi bwakuze bukoresha ibirahuri 8 byikirahure (ubunini bwa 55 × 45 × 50cm), hamwe nuburebure bwamazi bwa 40cm, ubushyuhe bwamazi karemano, hamwe nifaranga rihoraho. Amafi yubushakashatsi yashizweho kubushake hanyuma agabanywamo amatsinda abiri yo kugerageza. Itsinda rya mbere rigizwe na aquarium enye, zifite numero X1 (itsinda rishinzwe kugenzura), X2 (ibiryo 0.1gDMT / kg), X3 (ibiryo 0.2gDMT / kg), X4 (ibiryo 0.3gDMT / kg); Irindi tsinda rya 4 aquarium, ifite numero Y1 (itsinda rishinzwe kugenzura), Y2 (0.10g DMPT / kg ibiryo), Y3 (0.2g DMPT / kg ibiryo), Y4 (0.30g DMPT / kg ibiryo). Amafi 20 kuri buri gasanduku, agaburirwa inshuro 3 kumunsi saa 8h00, 13h00, na 17h00, hamwe nigaburo rya buri munsi rya 5-7% byuburemere bwumubiri. Ubushakashatsi bumaze ibyumweru 6. Ubushakashatsi butangiye kandi burangiye, hapimwe uburemere bw’amafi yipimishije kandi igipimo cyo kubaho kwa buri tsinda cyaranditswe.

2.1 Ingaruka zo kugaburira DMPT naDMTkuri karp
Ingaruka yo kugaburira DMPT naDMTkuri carp bigaragazwa ninshuro yo kuruma amafi yubushakashatsi mugihe cyiminota 2, nkuko bigaragara mu mbonerahamwe ya 1. Ubushakashatsi bwerekanye ko nyuma yo kongeramo ibiryo bya DMPT na DMT muri aquarium, amafi yubushakashatsi yahise agaragaza imyitwarire ikora yo kurisha, mugihe iyo akoresheje ibiryo byamatsinda yo kugenzura, reaction y amafi yubushakashatsi yari atinze. Ugereranije n'ibiryo bigenzura, amafi yubushakashatsi yagize ubwiyongere bugaragara inshuro zo kuruma ibiryo byubushakashatsi. DMT na DMPT bifite ingaruka zikomeye zikurura carp zigeragezwa.

Igipimo cyo kwiyongera kwibiro, umuvuduko wubwiyongere bwihariye, nigipimo cyo kubaho kwa karpi yagaburiwe hamwe nubushakashatsi butandukanye bwa DMPT yariyongereye cyane ugereranije nabagaburiwe ibiryo bigenzura, mugihe coefficient yibiryo yagabanutse cyane. Muri bo, kwiyongera kwa DMPT kuri T2, T3, na T4 byongereye ibiro bya buri munsi by’amatsinda atatu ku kigero cya 52,94%, 78.43%, na 113.73%, ugereranije n’itsinda rishinzwe kugenzura. Ibipimo byo kongera ibiro bya T2, T3, na T4 byiyongereyeho 60.44%, 73,85%, na 98.49%, naho umuvuduko w’ubwiyongere wiyongereyeho 41.22%, 51.15%, na 60.31%. Ikigereranyo cyo kubaho cyiyongereye kiva kuri 90% kigera kuri 95%, naho coefficient zagabanutseho 28.01%, 29.41%, na 33.05%.

3. Umwanzuro

Muri ubu bushakashatsi, nibaDMTcyangwa DMPT yongeyeho, inshuro yo kugaburira, umuvuduko wihariye wubwiyongere, hamwe no kongera ibiro bya buri munsi byamafi yubushakashatsi muri buri tsinda byariyongereye cyane ugereranije nitsinda rishinzwe kugenzura, mugihe coefficient yibiryo yagabanutse cyane. Niba ari DMT cyangwa DMPT, ingaruka zo gukura zigenda ziyongera cyane hamwe no kwiyongera kwinyongera mubyiciro bitatu bya 0.1g / kg, 0.2g / kg, na 0.3g / kg. Muri icyo gihe, hakozwe igereranya ryo kugaburira no gukura biteza imbere ingaruka za DMT na DMPT. Byagaragaye ko mu gihe kimwe cyo kogosha imisatsi, inshuro yo kugaburira, umuvuduko w’ibiro, n’umuvuduko wihariye w’amafi y’ubushakashatsi mu itsinda ry’ibiryo bya DMPT wariyongereye cyane ugereranije n’itsinda ry’ibiryo rya DMT, mu gihe coefficient yo kugaburira yagabanutse cyane. Ugereranije, DMPT ifite ingaruka zikomeye mukureshya no guteza imbere imikurire ya carp ugereranije na DMT. Ubu bushakashatsi bwakoresheje DMPT na DMT bwongewe kubiryo bya karp kugirango bigenzure ibiryo byabo no gukura biteza imbere ingaruka. Ibisubizo byerekana ko DMPT na DMT bifite amahirwe menshi yo gukoreshwa nkigisekuru gishya gikurura inyamaswa zo mu mazi.